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A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the separation and quantification of the enantiomers of N -methylaspartate and N -methylglutamate, after derivatization with N α  -(5-fluoro-2,4-dinitrophenyl)-L-leucinamide was established. The time required for the LC-ESI-MS/MS analysis was within 20 min and the detection limit was approximately 10 fmol per injection, demonstrating that this method can be used for the rapid determination of D-aspartate N -methyltransferase activity in the ark shell clam Scapharca broughtonii . Abbreviations: NMDA: N -methyl-D-aspartate; NMLA: N -methyl-L-aspartate; NMDG: N -methyl-D-glutamate; NMLG: N -methyl-L-glutamate; NMA: N -methylaspartate; NMG: N -methylglutamate; HPLC: high-performance liquid chromatography; SAM: S -adenosyl-L-methionine; OPA: o -phthalaldehyde; LC-ESI-MS/MS: liquid chromatography-electrospray ionization-tandem mass spectrometry; FDLA: N  α -(5-fluoro-2,4-dinitrophenyl)-L-leucinamide; FDAA: N  α -(5-fluoro-2,4-dinitrophenyl)-L-alaninamide; ESI: electrospray ionization; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry; MS/MS: tandem mass spectrometry.

bioscience, biotechnology, and biochemistry

Liquid chromatography-electrospray ionization-tandem mass spectrometric assay for <scp>d</scp>-aspartate <i>N</i>-methyltransferase activity in ark shells

Liquid chromatography-electrospray ionization-tandem mass spectrometric assay for d-aspartate N-methyltransferase activity in ark shells