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SpyTag and SpyCatcher can spontaneously and rapidly conjugate to form an irreversible and stable covalent bond. The trehalose synthase (TreS) from Thermomonospora curvata was successfully cyclized after the fusion of a SpyTag to its C-terminus and SpyCatcher to the N-terminus. Cyclized TreS retained more than 85% of its activity at temperatures ranging from 40 to 50°C and more than 95% at a pH range of 8 to 10, while the wild type kept only 60 and 80% of its activity under the same conditions. These results demonstrated that cyclized TreS had better resistance to high temperature and alkali than the wild type. Furthermore, structural analysis revealed that cyclized TreS had better conformational stability and was able to fold correctly at a higher temperature than the wild type. Our findings indicate that the use of SpyTag and SpyCatcher to cyclize enzymes is a promising strategy to increase their stability.

Enhancing the stability of trehalose synthase via SpyTag/SpyCatcher cyclization to improve its performance in industrial biocatalysts