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We evaluated the effect of whey protein hydrolysates (WPH) on the water absorption rate in the small intestine using a rat small intestine perfusion model. The rate was significantly higher with 5 g/L WPH than with 5 g/L soy protein hydrolysates or physiological saline (p &amp;lt; 0.05). WPH dose-dependently increased the water absorption rate in the range of 1.25–10.0 g/L. WPH showed a significantly higher rate than an amino acid mixture whose composition was equal to that of WPH (p &amp;lt; 0.05). The addition of 4-aminomethylbenzoic acid, an inhibitor of PepT1, significantly suppressed WPH’s enhancement of water absorption (p &amp;lt; 0.05). The rate of water absorption was significantly correlated with that of peptides/amino acids absorption in WPH (r = 0.82, p &amp;lt; 0.01). These data suggest that WPH have a high water absorption-promoting effect, to which PepT1 contributes.

Whey protein hydrolysates enhance water absorption in the perfused small intestine of anesthetized rats