Open AccessPyroglutamic acid stimulates DNA synthesis in rat primary hepatocytes through the mitogen-activated protein kinase pathway
Author(s) -
Shinjiro Inoue,
Yoichi Okita,
Andreia de Toledo,
Hiroyuki Miyazaki,
Eiichi Hirano,
Tetsuo Morinaga
Publication year - 2014
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1080/09168451.2014.991689
Subject(s) - pyroglutamic acid , protein kinase a , mapk/erk pathway , dna synthesis , kinase , biochemistry , hepatocyte , mitogen activated protein kinase , chemistry , microbiology and biotechnology , dna , biology , amino acid , in vitro
We purified pyroglutamic acid from human placental extract and identified it as a potent stimulator of rat primary hepatocyte DNA synthesis. Pyroglutamic acid dose-dependently stimulated DNA synthesis, and this effect was inhibited by PD98059, a dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) inhibitor. Therefore, pyroglutamic acid stimulated DNA synthesis in rat primary hepatocytes via MAPK signaling.
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