Open AccessPinctada fucata mantle gene 4 (PFMG4) from pearl oyster mantle enhances osteoblast differentiation
Author(s) -
Xiaoyan Wang,
Kenichi Harimoto,
Ryosuke Fuji,
Jing Liu,
Liyuan Li,
Pan Wang,
Toshihiro Akaike,
Zhao Wang
Publication year - 2014
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1080/09168451.2014.987206
Subject(s) - pinctada fucata , osteoblast , mantle (geology) , pearl oyster , microbiology and biotechnology , chemistry , alkaline phosphatase , biology , biochemistry , pearl , enzyme , in vitro , theology , paleontology , philosophy
The organic matrix of nacre has been reported for its effect on osteogenesis. It was found that PFMG4 (Pinctada fucata mantle gene 4) with an N-terminal signal peptide could be secreted into nacre of Pinctada fucata (P. fucata). Here, we report that PFMG4 is highly expressed in mantle tissue and has high homology with C1q protein in different species. In MC3T3-E1 osteoblast cells, we found that highly expressed PFMG4 could suppress cell proliferation and type I collagen expression, but it could increase alkaline phosphatase activity and mineralized deposition. These results show that PFMG4 has potential ability in enhancing osteoblast differentiation, suggesting a new idea in developing medicine for the therapy of osteoporosis.
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