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An immunoinformatics-based strategy is being investigated to identify prospective multi-subunit vaccine candidates against  Cryptosporidium hominis  ( C. hominis ). We used a systematic technique based on protein structure to create a competent multi-subunit vaccine candidate against  C. hominis , with the likelihood of antigenicity, allergenicity, and transmembrane helices as the screening criteria. Using the suitable linkers, the best-screened epitopes such as B-cell epitopes (BCL), Helper T-lymphocytes (HTL), and cytotoxic T-lymphocytes (CTL) were linked together to intensify and develop the presentation and processing of the antigenic molecules. The greatest 3 D model of the component protein was created with the help of modeling software called Raptorax. The validation of the modeled protein was accomplished via the use of PROCHECK. Furthermore, using the ClusPro web server, the projected modeled structure was docked with known receptor TLR-4 to determine their interactions. A molecular dynamics (MD) simulation was used to investigate the stability of the multi-subunit vaccine bound with TLR-4 based on the docking score. Aside from that, the codon optimization and  in silico  expression demonstrate the possibility of high expression and simple purification of the vaccine product resulting from codon optimization. The overall findings indicated that the multi-subunit vaccine might be a viable vaccination candidate against  C. hominis. Communicated by Ramaswamy H. Sarma

journal of biomolecular structure and dynamics

A multi-epitope vaccine candidate developed from unique immunogenic epitopes against  Cryptosporidium hominis  by utilizing an immunoinformatics-driven approach