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The antibacterial and antifungal effects of non-functionalized and surface-functionalized Mn 3 O 4  nanoparticles were comparatively analyzed to reason out the potential changes in antimicrobial activities due to functionalization with 5-amino-2-mercapto benzimidazole (AMB) molecule. The surface functionalization of Mn 3 O 4  nanoparticles with AMB molecule was confirmed by the XRD result, which shows the shift in 2 θ  values with noticeable peaks. The surface morphology and functionalization of Mn 3 O 4  nanoparticles were additionally confirmed by HR-SEM and EDAX studies. Antimicrobial activities were investigated by an agar-well-diffusion method using the bacteria  Staphylococcus aureus  and  Pseudomonas aeruginosa  and fungi  Aspergillus niger . The functionalized Mn 3 O 4  nanoparticles possess remarkable antibacterial and antifungal effect than the non-functionalized Mn 3 O 4  nanoparticles and AMB molecule. The coating of low energy surface layer over metal oxide nanoparticles such as Mn 3 O 4  offers an active surface toward both transfer of electron and the adsorption or desorption of water, inorganic ions, and other molecules, which leads to the increased antimicrobial activity of f-Mn 3 O 4  nanoparticles. Communicated by Ramaswamy H. Sarma

Mn 3 O 4 nanoparticles bearing 5-amino-2-mercapto benzimidazole moiety as antibacterial and antifungal agents