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Harmine exhibits pH dependent structural equilibrium and possesses numerous biological and pharmacological activities. Mode and mechanism of DNA binding and its cytotoxicity were studied by multiple spectroscopic, calorimetric, molecular docking and  in vitro  apoptotic as well as  in vivo  biochemical and histological studies. It exists as cationic (structure I) and decationic form (structure II) in the pH range 3.0–7.8 and 8.5–12.4, respectively, with a p Ka  of 8.0. Structure I at pH 6.8 binds strongly to DNA with a cooperative mode of binding of  K i ω  1.03 × 10 6  M −1 and stoichiometry of 5.0 nucleotide phosphates. Structure I stabilized DNA by 10 °C, showed85%quenching of fluorescence intensity, perturbation in circular dichroism, partial intercalation and enthalpy driven exothermic binding. While, structure II at pH 8.5 has very weak interaction with CT DNA. Cytotoxic potencies of structure I was tested on four different cancer cell lines along with normal embryonic cell. It showed maximum cytotoxicity with GI 50 of 20 µM, against HeLa causing several apoptotic induction abilities. Harmine exhibited G2M arrest with ROS induced effective role in PARP mediated apoptosis as well as anti-inflammatory action on HeLa cells. Harmine further presented MIC and antibiofilm activity against  Staphylococcus aureus  in presence of &lt;160 and 30 µg/ml, respectively. Mice with post harmine treatment (30 mg/kg b.w., I.P.) showed maximum recovery from damaged to near normal architecture of cervical epithelial cells. This study may be of prospective use in a framework to design novel beta carboline compounds for improved therapeutic applications in future against cervical cancer.HighlightsHarmine exists in structure I and structure II forms in the pH 6.8 and 8.5with a p Ka  of 8.0.Structure I at pH 6.8 binds strongly to DNA compared to structure II.Structure I showed maximum cytotoxicity with GI 50  of 20 µM against HeLa.ROS mediated cytotoxicitywithG2M arrest with PARP mediated apoptosis was studied.Harmine (30µg/ml) exhibited antibiofilm activity against  Staphylococcus aureus .Post harmine dose (30 mg/kg b.w., I.P.) in mice showed recovery of cervical epithelial cells. Harmine exists in structure I and structure II forms in the pH 6.8 and 8.5with a p Ka  of 8.0. Structure I at pH 6.8 binds strongly to DNA compared to structure II. Structure I showed maximum cytotoxicity with GI 50  of 20 µM against HeLa. ROS mediated cytotoxicitywithG2M arrest with PARP mediated apoptosis was studied. Harmine (30µg/ml) exhibited antibiofilm activity against  Staphylococcus aureus . Post harmine dose (30 mg/kg b.w., I.P.) in mice showed recovery of cervical epithelial cells. Communicated by Ramaswamy H. Sarma

journal of biomolecular structure and dynamics

Structure-activity insights of harmine targeting DNA, ROS inducing cytotoxicity with PARP mediated apoptosis against cervical cancer, anti-biofilm formation and  in vivo  therapeutic study