English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Rhodococcus  bacteria are a fast-growing platform for biocatalysis, biodegradation, and biosynthesis, but not a platform for molecular biology. That is,  Rhodococcus  are not convenient for genetic engineering. One major issue for the engineering of  Rhodococcus  is the absence of a publicly available, curated, and commented collection of sequences of genetic parts that are functional in biotechnologically relevant species of  Rhodococcus  ( R. erythropolis ,  R. rhodochrous ,  R. ruber , and  R. jostii ). Here, we present a collection of genetic parts for  Rhodococcus  (vector replicons, promoter regions, regulators, markers, and reporters) supported by a thorough analysis of their functionality. We also highlight and discuss the gaps in  Rhodococcus -related genetic parts and techniques, which should be filled in order to make these bacteria a full-fledged molecular biology platform independent of  Escherichia coli . We conclude that all major types of required genetic parts for  Rhodococcus  are available now, except multicopy replicons. As for model  Rhodococcus  strains, there is a particular shortage of strains with high electrocompetence levels and strains designed for solving specific genetic engineering tasks. We suggest that these obstacles are surmountable in the near future due to an intensification of research work in the field of genetic techniques for non-conventional bacteria.

Rhodococcus : sequences of genetic parts, analysis of their functionality, and development prospects as a molecular biology platform