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The placenta-derived extracellular matrix is an ideal material for making freeze-dried sponges as a wound dressing. In order to prepare a porous sponge, collapse temperature is one of the most important parameters in process of freeze-drying. This work used an image processing method to determine the collapse temperature of extracellular matrix slurry during freeze-drying. By using this technology, the variation of collapse temperature under different cooling rates, heating rates, annealing conditions are studied, and the influence of ice crystal size on collapse temperature is further analyzed. In addition, an annealing operation is used to control the size of ice crystals inside the sample. The actual results are consistent with the fact that annealing increases the ice crystal size and collapse temperature. The image processing algorithm can greatly improve the accuracy of obtaining the collapse temperature to shorten the period of exploration of the key freeze-drying parameters of solid-liquid mixtures.

drying technology

Acquisition of collapse temperature and the influencing factors during freeze-drying of placental decellularized matrix in freeze-drying microscopy based on image processing techniques