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To explore the effects of essential amino acid (EAA) supplementation on high-intensity interval training (HIIT) fatigue, perceived exertion, and training progression in overweight and obese adults. A secondary aim was to explore potential sex-differences on these outcomes. Thirty-seven untrained adults (51% female; 36.2 ± 5.9 yrs; 35.5 ± 6.7% body fat) completed eight weeks of HIIT, 2d/wk on a cycle ergometer, either with EAA supplementation (HIIT + EAA; 3.6 g of EAA twice daily, 30 minutes pre and post HIIT) or without supplementation (HIIT). Heart rate (HR) and ratings of perceived exertion (RPE) were recorded throughout each session as indices of within training fatigue. Time to exhaustion (TTE) was recorded for the final interval of each session. Workload progression was determined by change in watts. Differences between groups (with and without EAA) were evaluated at 1wk, 4wks, and 8wks by repeated measure ANOVAs (α = 0.05). There were no differences in TTE ( p  = 0.983) or workload progression ( p  = 0.655) with EAA supplementation at any time point. HR and RPE within HIIT sessions were not significantly different with EAA supplementation at any time point ( p  &gt; 0.05). Results were similar when evaluating males and females separately, but in females, RPE was significantly lower with EAA supplementation at 4wks (Δ: 1.1-2.2;  p  = 0.016). EAA supplementation did not extend TTE during exercise or enhance workload progression across eight weeks of HIIT in untrained, overweight and obese adults. However, EAA consumed 30 minutes before exercise may reduce perceived exertion during the first four weeks of training in women, which may have implications for overall exercise enjoyment and long-term adherence.

Effects of Essential Amino Acids on High-Intensity Interval Training Performance, Fatigue Outcomes, and Workload Progression