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In this research, the effectiveness of electrical pulse-mediated  Piper nigrum  (Black pepper) extracts against MCF-7, ER-positive human breast cancer cells were investigated. Initially,  P. nigrum  extracts were extracted by using the conventional (Soxhlet) and electrical pulse-assisted method. We assessed the phytochemicals present in both extracts by phytochemical screening tests and compared their total phenol contents and antioxidant activity with the conventional extract. FTIR and GCMS for untreated and treated extracts were also measured and compared. Electrically enhanced extracts (EE) resulted in an improvement of ∼41% in its antioxidant activity and ∼24% increase in their total phenol contents than untreated extracts. The cytotoxic activity of both extracts was determined by MTT proliferation assay in a dose-dependent manner. The results show that electrical pulse-assisted extracts have enhanced the bioactive compounds, with respect to untreated extracts, significantly promoted total phenols and antioxidant activity, which is correlated with increased MCF7 cytotoxicity. Furthermore, to enhance the uptake of extracts against plasma membranes, electrical pulses of different parameters (EP1 and EP2) were used. The results indicate cell viabilities of 27% with raw extract only, 12% with EE only, 7% with (EP1 + EE) and 6% (EP2 + EE) after 72h, indicating the potential of the synergetic effect of electrical pulses (EP) and enhanced extract (EE). On comparison, the enhanced extract showed viability of two times less than raw extracts, whereas when it is mediated with electrical pulses, there is a marked reduction in cell viability. This clearly indicates that the application of electrical pulses aids in achieving antioxidant and phenolic-rich extracts. Furthermore, this treatment is more appropriate to achieve maximum cell death, with less concentration of the extract to treat breast cancer.

Synergy of Electrical Pulses and Black Pepper ( Piper nigrum ) Extracts for Effective Breast Cancer Treatment: An In Vitro Model Study