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The contamination of brewers’ spent grains (BSG) with mycotoxins may pose a significant threat to the health of domestic animals that consume BSG as feed. New information is needed regarding the presence of mycotoxins throughout commercial beer and BSG production. Samples ( n  = 106) were collected during production of a single batch of commercial beer, including malt ( n  = 54), mash ( n  = 6), wort ( n  = 6), fermentation, ( n  = 14), beer ( n  = 3), and BSG ( n  = 23) and analyzed for the presence of deoxynivalenol (DON), 3-acetyldeoxnivalenol (3ADON), 15-acetyldeoxynivalenol (15ADON), and zearalenone (ZEN) using gas chromatography–mass spectrometry (GC-MS). DON, 3ADON, 15ADON, and ZEN were observed at low levels (&lt;0.05 mg kg −1 ) throughout the production of the beer. Despite most of the samples having low levels of mycotoxins, qualitative analyses showed trace levels of DON, 15ADON, and ZEN in barley malt, solid-fractions of mash, and BSG. Overall, results from this study suggest that low-levels of mycotoxins are retained in BSG, which may consumed by livestock. Results may help brewers and animal producers to consider storage and feeding procedures for BSG.

Tracking Zearalenone and Type-B Trichothecene Mycotoxins in the Commercial Production of Beer and Brewers’ Spent Grains