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Rosa roxburghii  is a medicinal and edible plant, which is favored by consumers due to its rich vitamin C content. Residues and potential health risks of difenoconazole in the  R. roxburghii  ecosystem has aroused a concern considering its extensive use for controlling the powdery mildew of  R. roxburghii . In this study, the residue of difenoconazole in  R. roxburghii  and soil was extracted by acetonitrile, purified by primary secondary amine and detected by liquid chromatography-tandem triple quadrupole mass spectrometry. The average recoveries in  R. roxburghii  and soil matrix varied from 82.59% to 99.63%, with relative standard deviations ( RSD ) of 1.14%–8.23%. The limit of quantification ( LOQ ) and detection ( LOD ) of difenoconazole in  R. roxburghii  and soil samples were 0.01 mg/kg. The dissipation of difenoconazole followed well the first-order kinetic, with a half-life of 3.99–5.57 d in  R. roxburghii  and 4.94–6.23 d in soil, respectively. And the terminal residues were &lt;0.01–2.181 mg/kg and 0.014–2.406 mg/kg, respectively. The chronic and acute risk quotient values of difenoconazole were respectively 0.42% and 4.1%, which suggests that the risk was acceptable and safe to consumers. This study provides a reference for the safe and reasonable use of difenoconazole in  R. roxburghii  production.

Dissipation, residue and dietary risk assessment of difenoconazole in Rosa roxburghii