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Caffeine content is a crucial attribute of coffee. Its concentration and thus maximum cups of  Coffea arabica  from Africa, Asia, Central America, and South America from different altitudes of growing areas, altitude, and process using different post-harvest processing (dry, wet, and pulped natural). Our results suggest that geographical origin might affect the alkaloid concentration in  C. arabica . The caffeine concentration pattern in green samples was as follows: Central America &gt; South America &gt; Asia &gt; Africa. Altitude affected the concentrations, lowlands &gt; midlands &gt; highlands, however, not significantly. Given caffeine is thermostable, the medium roasting process did not affect the concentration of caffeine directly, but a small increase was observed. Scientific opinion on the safety of habitual caffeine consumption of up to 400 mg per day does not raise safety concerns for non-pregnant adults. A cup (7 g coffee in 120 mL of water) was used for recalculation. Results suggest that mostly highlands and midlands coffee from Africa reached levels of caffeine that might be consumed in more than 5.5 cups a day.

journal of environmental science and health, part b

Variability of caffeine content in green and roasted  Coffea arabica  regarding the origin, post-harvest processing, and altitude, and overview of recommended daily allowance