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In order to understand the use of micro farming specific local bio-control agents against the foliar pathogens, twenty-one  Trichoderma  isolates were collected from different cropping locations of Karnataka. In morphological characterization: all colonies were appeared dark green with flat, even/uneven growth with 2–4 concentric rings and sporulation was observed after 2–4 days after inoculation on potato dextrose agar medium. The length × width of conidia and phialides varied from 2.5–4.6 μ m  × 2.5–4.6 μm and 6–11.3 μ m  × 2–3.5 μm respectively. In Basic Local Alignment Search Tool analysis of 5.8S-ITS region was amplified using ITS-4 and 5. These results assisted in species identification and classified into five clades ( T. harzianum : 11,  T. asperellum ; 5,  T. virde ; 2,  T. virens ; 2 and  T. longibrachiatum ; 1). Further, in antagonistic activity, RMlO exhibited highest growth inhibition of  Asperisporium caricae  (96.30%),  Pyricularia oryzae  (97.03%) and  Bipolaris oryzae  (100%)) significantly over the commercial isolate ( A. caricae  (85.93%);  P. oryzae  (89.38%) and  B. oryzae  (91.92%)).

Micro-farming specific morpho-molecular characterization of Trichoderma isolates and its bio-control potential against foliar fungal plant pathogens