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A study was conducted to isolate efficient biocontrol agent against agriculturally important plant pathogens. Isolated fungus was identified using morphological and molecular characters and evaluated against plant pathogens. Isolated culture was identified as  Trichoderma longibrachiatum  based on morphological and cultural characteristics. The isolate (TL-RD-01) was able to solubilize the zinc and able to produce the indole 3 acetic acid of about 32.01 μg/ml. To optimize the growth media different carbon and nitrogen sources were studied. Maltose, Corn flour, Peptone and Yeast Extract used media attained maximum growth of  T. longibrachiatum.  The pH (5 to 6) and temperature (25 °C to 30 °C) was optimized for the growth of  T. longibrachiatum  TL-RD-01. Molecular identification based on internal transcribed spacer 1 and 2 region (ITS1 and ITS2) sequence analysis have been further confirmed as  T. longibrachiatum . The phylogenetic analysis of above ITS gene sequences were carried out.  T. longibrachiatum  isolate was clearly separated with ITS sequence’s analysis with other species of  Trichoderma.  Mycoparasitic effect of  T. longibrachiatum  was carried out by dual plate technique against agriculturally important plant pathogens  viz .,  Macrophomina phaseolina, Phytopthora infestans, Collectrotrichum gleosporides  (Mango) and  Colletotrichum falcatum.  Pathogen inhibition effect of  T. longibrachiatum  isolate was in between 50% to 81.94%. As per the investigation,  Trichoderma longibrachiatum  (TL-RD-01) could be deployed as a biocontrol agent and a biostimulant.

Characterization and biocontrol potential of Trichoderma longibrachiatum TL-RD-01 against plant pathogens