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Symptoms of severe leaf curling, typical of begomovirus infection, were observed on a plant of  Brugmansia arborea  grown in lawns of Quaid-i-Azam university Islamabad, Pakistan. A begomovirus and its associated betasatellite were amplified and sequenced from infected plants. Nucleotide sequence showed that a monopartite begomovirus named  Chilli leaf curl virus  (ChLCV) and  Chilli leaf curl betasatellite  (ChLCB) are associated with the disease and it shared highest sequence identity 99% and 91% respectively with previously reported clones. Our data adds to recent reports that show an expansion of the host range of begomovirus-betasatellite complexes in the Old World.

Brugmansia arborea reported as a new host of a begomovirus and betasatellite from Pakistan