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A sensitive photoelectrochemical biosensor was constructed for the detection of chlorpyrifos. The sensor was prepared on indium tin oxide (ITO) electrode using photoelectric material bismuth vanadate (BiVO 4 ) and acetylcholinesterase (AChE). It is found that the photocurrent of ITO/BiVO 4  electrode was largely dependent on its treatment temperature. The highest anodic photocurrent response of the ITO/BiVO 4  photoelectrode was obtained after the treatment at 300°C. The thiocholine, produced in the catalytic reaction at ITO/BiVO 4 /AChE electrode surface, can greatly enhanced the anodic photocurrent. In addition, the adsorption of chlorpyrifos on the surface of the AChE caused a decrease in the intensity of the photocurrent. Under the optimisation of experimental conditions, the decrease of the photocurrent showed a good linear range from 1 pM to 10 nM under irradiation of visible light. The detection limit is calculated as 0.25 pM with a 10% inhibition rate. The ITO/BiVO 4 /AChE photoelectrode exhibited high sensitivity, acceptable stability and good reproducibility. The biosensor can be provided to the potential applications for detection of pesticide residues in food.

A sensitive photoelectrochemical biosensor for pesticide detection based on BiVO 4