English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A simple magneto immunofluorescence assay for quinolone family detection in 96-well microplate was developed and applied to milk and groundwater samples. Norfloxacin (NOR) conjugated to albumin (BSA) was immobilised on magnetic beads (MBs) in a first step. The strategy was based on an indirect competitive assay between free and immobilised quinolone for anti-quinolone IgG antibody. Then, a peroxidase tracer was added as developer reagent. The fluorescence responses at 440 and 540 nm - excitation and emission wavelengths, respectively – were obtained by adding o-phenylenediamine and peroxide hydrogen as peroxidase substrates. The fluorescence response was indirectly proportional to the concentration of quinolone in the sample. Ciprofloxacin was selected as model quinolone with cut-off and limit of detection (LOD) of 4 and 8 μg L −1 , respectively. Bovine milk and groundwater blank samples were spiked with ciprofloxacin at 5, 25 and 50 µg L −1  obtaining good recoveries (90–100%) with coefficient variation lower than 20% in all cases, in concordance with the results obtained by applying a reference method. Therefore, the method developed would be applicable for the detection of quinolones in bovine milk from dairy farms and in groundwater from sites with intensive livestock activities.

Detection of quinolones in milk and groundwater samples using an indirect immunofluorescent magneto assay