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Metronidazole was traced by a new antibiotic ampicillin sodium based-carbon quantum dots (AMP N-CDs) according to fluorescence quenching mechanism. It had been found in the experiment that through dual effects of static quenching and internal filtration, the fluorescence of AMP N-CDs could be quenched by Metronidazole. Based on this phenomenon, we had established a fluorometric method to detect Metronidazole in water samples with fluorescent CDs. Metronidazole concentration showed good linear relationship with fluorescence quenching efficiency in the range of 20–100 μM (R = 0.9919) and the limit of detection (LOD) was 65.2 nM. Through these results, the AMP N-CDs was successfully used to detect Metronidazole in real samples. It provided a new insight on the bioimaging application and environmental pollutants detection.

Application of Metronidazole detection by antibiotic ampicillin sodium based-carbon quantum dots