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It is necessary to delignify wood samples and treat them with ethylene diamine (EDA) before they are dissolved in 8% (w/v) lithium chloride/ N , N -dimethylacetamide (LiCl/DMAc) prior to size-exclusion chromatography (SEC) analysis. In the present study, the effects of delignifying birch wood powder 0–3 times with NaClO 2  and subsequently treating it with EDA on its solubility in 8% (w/v) LiCl/DMAc and its SEC data. The neutral sugar composition of the birch powder was almost unaffected by either delignification or treatment with EDA. Treatment of the birch powder with EDA resulted in 28% solubilities in 8% (w/v) LiCl/DMAc. Approximately 11% of cellulose molecules in the birch wood powder was dissolved, and detected as a high-molar-mass (HMM) fraction in the SEC elution pattern. Each single delignification treatment increased the solubility in 8% (w/v) LiCl/DMAc to 68–74% after EDA treatment. Based on the glucose contents of the delignified samples, almost all cellulose molecules in the delignified samples were dissolved in 8% (w/v) LiCl/DMAc after EDA treatment, and detected as the HMM fractions in the SEC elution patterns. The HMM cellulose molecules in the EDA-treated birch powder had linear random-coil conformations in 1% (w/v) LiCl/DMAc. However, the SEC data suggest that there probably were some chemical linkages between the HMM cellulose molecules and lignin or NaClO 2 -treated lignin fragments in the HMM fractions.

Changes in chemical structures and molar mass parameters of birch wood powder by ethylene diamine treatment