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Structured lipid microparticles (StLMs) composed of oil and solid lipid in specific ratios are drawing significant attention, owing to their stability in loading and controllability in releasing bioactive compounds. In this study, we formulated monodisperse StLMs with palmitic acid or tripalmitin mixed with soybean oil in different ratios using microchannel emulsification (MCE) at ∼70 °C and following crystallization by cooling. Based on the physicochemical properties of the different dispersed phases, the formulation characteristics were observed and investigated using a microscope video system connected to the MCE system. For each ratio and composition at ∼70 °C, the lipid droplets presented average diameter and coefficient of variation (CV) values in respective ranges of 15.5–19.0 μm and 3.3–8.4%. Following droplet crystallization by cooling, the StLMs exhibited morphological changes but negligible changes in average size and CV. From the internal structure of each lipid molecular and the fundamental information supplied by differential scanning calorimetry (DSC) experiments, we outline the assumptions for morphology variation, which can be used as reference for encapsulation research of StLMs.

Formulation characteristics of monodisperse structured lipid microparticles using microchannel emulsification