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This study aimed to formulate the green, sustainable, and ecofriendly nanobiopesticides of  Azadirachta indica  with enhanced pest control efficacy. Nanoprecipitation method was used for the development of nanobiopesticides. Optimisation was done by response surface methodology. Nanoformulations were characterised by zetasizer, scanning electron microscopy, energy dispersive x-ray spectroscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. Pesticidal potential of nanosuspensions was evaluated by insecticide impregnated filter paper method. Optimised nanobiopesticide showed an average particle size of 275.8 ± 0.95 nm, polydispersity index (PDI) 0.351 ± 0.002, and zeta potential of –33 ± 0.90 mV. Nanobiopesticides exhibited significantly higher mortality rates of 86.81 ± 3.04 and 84.97 ± 2.83% against  Tribolium castaneum  and  Ryzopertha dominica , respectively, as compared to their crude extract. Minor change in particle size from 275.8 ± 0.95 to 298.8 ± 1.00 nm and PDI from 0.351 ± 0.002 to 0.445 ± 0.02 were observed after 3 months of storage at 4 °C. Pesticidal efficacy of  A. indica  was significantly enhanced by the formulation of its nanobiopesticides.

journal of microencapsulation

Formulation and characterisation of  Azadirachta indica  nanobiopesticides for ecofriendly control of wheat pest  Tribolium castaneum  and  Rhyzopertha dominica