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Nitrogen (N) is the nutrient most used by crop producers and its application may affect the N use efficiency in crop production. The present study was conducted in order to determine the optimum N fertiliser rate and application stages (S) for stevia ( Stevia rebaudiana  Bertoni) production for the first cutting, under greenhouse conditions. A two-factorial experiment with four N fertiliser rates (0, 40, 80 and 120 kg ha −1  applied as urea) and three stages (sole split N applied at sowing; dual split N applied at sowing and one month after sowing; triple split N applied at sowing, one and two months from sowing) were laid out in a completely randomised design. Each treatment combination was replicated three times. The plants were grown in boxes (1 m −2 , 40 cm high) filled with loam soil and the treatments were applied as described. Results showed that stevia growth and metabolites were significantly affected by N rates and application stages. Stevia leaf area index (LAI), leaf yield, leaf/stem ratio and harvest index (HI), steviol glycosides (SVglys) content, SVglys compositions and yield were the highest when 40 kg N ha −1  was applied in two stages. This was also true for leaf and SVglys yield. Splitting of N application of 120 kg ha −1  into three stages showed an inhibitory effect on some stevia traits such as SVglys content and yield. Based on the current results and efficient N application, 40 kg N ha −1  split into two stages is recommended for stevia production under greenhouse conditions.

Optimising the rate and stages of application of nitrogen fertiliser for stevia under greenhouse conditions