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Ozone gas (O 3 ) is used as an oxidizing agent and can be applied as a sanitizer to control microorganisms and degrade toxic compounds, such as pesticides and mycotoxins, in food industries. Some studies investigated the use of O 3  as an agent to reduce pathogenic bacteria such as  Salmonella enteritidis . The action of O 3  on the eggshell cuticle and structures of filamentous fungi is still not yet well studied. In this work, scanning electron microscopy (SEM) was used to assess the hens’ eggshell structure after the exposition to O 3 , mainly to obtain information about the potential antifungal action. The SEM micrographs obtained shown that the application of O 3  during 120 min damaged fungi hyphae adhered to the eggshell cuticle without severely compromising the external structure of the eggshell. Further studies are necessary to establish an ideal O 3  treatment for the fungal decontamination of chicken eggs.

Antifungal Action of Ozone on Chicken Eggshell Cuticles: A Preliminary Study