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The objectives of this study were to evaluate the toxicity of ozone on  Zabrotes subfasciatus  (Boheman, 1833) in lima bean seeds, determine the instantaneous population growth rate (r i ) of these insects exposed to lethal and sublethal ozone doses, and evaluate their effects on the physical and physiological characteristics of lima beans. Toxicity was determined by using estimates of lethal doses (mg/g) for 50% and 95% of adult insects (LD 50  and LD 95 ) at the top and bottom of the fumigation chamber. We found that LD 95  values were 121.75 and 87.79 mg/g when the insects were distributed at the top and bottom of the chamber, respectively. The r i  values decreased from 0.077 to 0.002 when the seeds were exposed to ozone, while they increased from 0.07 to 0.08 when the seeds were exposed to oxygen. The water content of the seeds decreased from 11.2% b.u to 10.38% b.u. when the ozone dose increased. The germination percentage increased from 67% to 93% when the ozone dose was increased. Conductivity decreased from 51.88 μS cm −1  g −1  to 32.47 μS cm −1  g −1  when the ozone dose increased. The apparent specific mass increased when the ozone dose increased from 732.44 m −3  to 738.51 m −3 . Ozone is toxic to  Z. subfasciatus  and affects its instantaneous growth rate; however, it does not affect grain quality. Therefore, ozone has the potential to be used to control  Z. subfasciatus  in stored grains.

ozone: science and engineering

Ozonation of Lima Bean (Phaseolus Lunatus L.): Control of Zabrotes Subfasciatus (Boheman, 1833) (Coleoptera: Chrysomelidae: Bruchinae) and Maintenance of Grain Quality