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Recent studies have recorded new species of freshwater planarians in caves of the biomes Amazonia, Atlantic Forest, Cerrado, and Caatinga. Herein we contribute to enhancing this knowledge by describing three new cave-dwelling species of  Girardia  from three different cave systems situated in two biomes (Atlantic Forest and Cerrado) in eastern Brazil.  Girardia spelaea  sp. n., from a limestone cave, is eyeless and shows a whitish body. The other two species,  G. asymmetrica  sp. n. and  G. ibitipoca  sp. n., from limestone and quartzite caves, show pigmented bodies and eyes. The three species are characterized by dorsal and/or dorsoventral testes and a bulbar cavity with forked ental portions, varying from round or ovoid to elongate. Each new species is easily recognized by a unique combination of characters of their external morphology and reproductive system.  Girardia spelaea  is probably a troglobitic species and the two other species may be trogrophiles, all of them with a restricted known distribution. urn:lsid:zoobank.org:act:AC4F9F3C-EF6D-4BB8-ACBA-EB5812036314urn:lsid:zoobank.org:act:6BD22CDA-99E7-46DC-98D6-8FD1ECB46320urn:lsid:zoobank.org:act:C5D97034-9F1A-4020-9635-DE76202A85C1

studies on neotropical fauna and environment

Enhancing the still scattered knowledge on the taxonomic diversity of freshwater triclads (Platyhelminthes: Dugesiidae) in caves from two Brazilian Biomes