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Rhipicephalus (Boophilus) microplus  has a strong impact on bovine health. The aim of this study was to evaluate the  in vitro  acaricidal activity of  Piper tuberculatum  extracts on  R. (Boophilus) microplus  ticks obtained from cattle. The adult immersion test (AIT) was used to estimate the inhibition of oviposition. Moreover, the larval package test (LPT) was performed.  P. tuberculatum  extracts at 100 mg/ml showed a mortality of 78% after 24 h in adult ticks, reaching 100% at 9 days. Moreover, the mortality rate was signicantly dierent (p˂0.001) among the tested groups at 11 days. Probit regression analysis showed that the mean lethal concentration (LC50) of  P. tuberculatum  extracts was 20.30 mg/ml after 24 h. On day 11, there were signicant dierences in oviposition rate among the groups, with 56% oviposition in the control group, whereas no oviposition was observed (p˂0.01) in the groups treated with  P. tuberculatum  at 100 mg/ml. In the LPT, high mortality (56.00%) was found in the group treated with  P. tuberculatum  (50 mg/ml), whereas the control group had 2.67% (p˂0.01). The use of  P. tuberculatum  extracts (100 mg/ml) was highly eective as an acaricide, reaching a high mortality rate in adult  R. microplus  ticks.

In vitro acaricidal activity of Piper tuberculatum against Rhipicephalus (Boophilus) microplus