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Anaerobic process is a promising technique for textile effluent treatment. However, poor solid–liquid separation and residual chemical oxygen demand (COD) in the treated effluent limit its scope for industrial applications. The study analyzed the nature of suspended microbial biomass in the anaerobically treated effluent and further targeted its feasible separation process. The size of the majority of suspended biomass (82%) particles ranged from 500 to 1000 nm. The suspended biomass showed 37.13% hydrophobicity and −15.4 mV zeta potential. The optimization of the coagulation-flocculation (CF) process showed 93% turbidity removal at 400 mg/L of alum, 8 mg/L of a cationic polymer and acidic pH 4, through one variable at a time approach. The optimized results using Central Composite Design (CCD) were experimentally validated, and 97% turbidity removal was recorded at 400 mg/L alum concentration, 12 mg/L of the cationic polymer, and pH 3.2. The results obtained were statistically significant, with a high regression coefficient (R 2  = 0.97) at 95% confidence limits. The final treated effluent after CF showed 67% COD removal (from 862 ± 40 to 280 ± 14 mg/L) with 83% color removal (from 740 to 124 Hazen). Hence, the integration of the CF process with the anaerobic process could be a promising approach for textile effluent treatment.

Integration of coagulation-flocculation process for improved solid–liquid separation of anaerobically treated textile effluent