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A simple and rapid syringe-to-syringe dispersive liquid-phase microextraction (SS-DLPME) technique has been developed for the separation and preconcentration of mercury combined with UV-Vis spectrophotometry. In this method, effective factors were screened using a Plackett–Burman design (PBD) and optimized using a Box–Behnken design (BBD). Under the optimized experimental conditions, the calibration curve was linear in the range of 5–400 µg L −1  with the coefficient of determination ( r2 ) of 0.997. The detection limit, intraday precision, inter-day precision and the enrichment factor (EF) were 1.60 μg L −1 , 1.57%, 1.82% and 120, respectively. This method was successfully applied to the determination of Hg(II) in water, fish and human hair samples.

separation science and technology

Application of robust syringe-to-syringe dispersive liquid-phase microextraction method for preconcentration and determination of mercury with the aid of an experimental design