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In this investigation, samples of mining dolomite wastes were collected from Abu Tartur phosphate mines, Egypt. The samples were thermally decomposed to MgO/CaO mixture as evidenced by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The MgO/CaO combination mixture was utilized in this study as an antibacterial agent against  Bacillus cereus ,  Staphylococcus aureus , and  Staphylococcus epidermidis . Antibacterial experiments have revealed that the MgO/CaO combination was more effective against  Bacillus cereus ,  Staphylococcus aureus , and  Staphylococcus epidermidis . For the three bacterial strains, the minimum inhibitory concentration (MIC) of the tested mixture was found to be 1.0 µg/mL. Acridine Orange pictures, which helped to explain the high occurrence of dead cells, and were utilized effectively to record the effect of MgO/CaO, which was employed in this investigation on the bacterial growth. Taking into consideration, MgO/CaO combination was found to be very interesting candidate material for bacterial disinfection due to its simplicity of synthesis, low cost, low release rate, and as excellent antibacterial activity.

Utilization of Mining Rock Wastes as Antibacterial Agents Against Gram Positive Bacteria