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In this work, we developed a simple spectrophotometric strategy for BrO 3−  ions determination as a major water disinfection constituents in the mice’s liver tissues by using pararosaniline (PRA). Mice were divided into seven main groups (6 doses): lowest dose KBrO 3  (G 1  0.01 mg L −1 , G 2  0.025 mg L −1  and G 3  0.1 mg L −1 ), highest dose KBrO 3  (G 4  1 mg L −1 , G 5  10 mg·L −1  and G 6  30 mg L −1 ) and control. All these groups maintained a dose-specific feeding for one month, just before the bromate assessment in mice's liver samples. The results revealed that groups of exposure to lower doses of drinking water did not detect the presence of BrO 3−  accumulated in the liver tissue during the study period (1–2 months). While, the BrO 3−  was detected in higher dosages for samples analyzed in first, second, third, fourth and fifth weeks (W 1 , W 2 , W 3 , W 4 , and W 5 ). These results confirmed that the higher BrO 3−  dosages (1, 10, and 30 mg L −1 ) were fatal if introduced in drinking water and could accumulate in the liver tissues both for mice and for human. Detection the accuracy of the method for recovery of bromate ions in liver samples ( N  = 5) was found to be more than 95%. Relative standard deviations (RSDs) were found to be less than 2.0% confirming the reproducibility of the assay technique.

Oral administration of a potassium bromate dosage: Determination and evaluation of accumulated bromate on the liver of male mice