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In this study, poly(lactic-acid) (PLA) polymer-based nanofiber (NF) was obtained by the electrospinning method. Antioxidant Zataria Multiflora (ZM, essential oil extract) and antibiotic effective bacitracin (BAC) materials were added to 8% PLA. ZM essential oil was extracted with Rotary and antioxidant components were investigated using GC-MS. While carvacrol constitutes 50% as the main component of ZM, it was seen as 48.06% carvacrol and 25.47% thymol as a content area. It was analyzed that ZM has a total antioxidant rate of 99.87% with 20 components. In antibacterial tests, PLA/BAC/ZM NF showed the best inhibition growth on all microorganisms ( Escherichia coli ,  Staphylococcus aureus ,  Bacillus cereus, Staphylococcus epidermidis ,  Bacillus subtilis ). In cell XTT cytotoxicity tests, it was observed that the PLA/BAC/ZM NF used in adhesion and 4′,6-diamidino-2-phenylindole (DAPI) results showed very good adhesion to cells.

Antibacterial and cellular behavior of PLA-based bacitracin and zataria multiflora nanofibers produced by electrospinning method