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A biopolymer film serving as a wound-healing substitute was fabricated using keratin and fibrin (extracted from a goat hoof and blood, respectively) via modified Shindai techniques. The importance of keratin and fibrin along with gelatin and mupirocin for the fabrication of a biofilm by solvent casting method is shown in this work.  In vitro  microscopic images of the fibroblast NIH 3T3 cell lines revealed the biocompatibility, cell viability, cell adhesion, and proliferation of biopolymer film in the wound site. The low-cost and biowaste-source-extracted keratin and fibrin blended biopolymer film supports the wound-healing process in humans.

Biopolymer film fabrication for skin mimetic tissue regenerative wound dressing applications