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Non-enzymatic glucose biosensors have attracted considerable attention due to their approved benefits. The present study aims to design and produced a non-enzymatic biosensor based on polyacrylonitrile/polyaniline/CuO nanofibrous composites on indium-tin oxide (ITO) substrate using single-step electrospinning at different conditions. Electron microscopic studies show that continuous and uniform fibers from an optimum blend of PAN/PANI (90/10 mass ratio) incorporating with 5% CuO were successfully fabricated under 20 kV and at 18 cm. By employing of FTIR and XRD, characteristic peaks of chemical and crystalline structures of polymers and CuO were detected, which indicate successful incorporation of CuO into the nanofibers. Electrochemical studies, including cyclic voltammetry followed by amprometry have also been performed to evaluate the performance of the biosensor. The prepared electrode showed a linear response (R 2  = 0.9983) over a wide concentration range from 1 × 10 −5  to 3 × 10 −3 M. The detection limit based on signal to noise ratio of 3 was 1.2 × 10 −6 M.

Novel non-enzymatic glucose biosensor based on electrospun PAN/PANI/CuO nano-composites