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Biomaterials and thin biofilms play a fundamental role in the medical, food and pharmaceutical industries. The Quartz crystal microbalance (QCM) measurement technique is one of the attractive techniques which have been used for monitoring the thin-film formation process. In the current work described here, κ-carrageenan and chitosan solutions were used for the layer-by-layer (LBL) deposition of polyelectrolyte multilayers (PEMs) on the gold surface of a 5 MHz AT-cut (thickness-shear mode) quartz crystal in a flow module. A sensitive QCM system was designed to detect 0.1 Hz differences in the resonance frequency ( Δf ) and 10 −7  changes in the energy dissipation ( ΔD ) values, which are the measures of the deposited mass and the film rigidity, respectively. Negatively charged carrageenan and positively charged chitosan biomolecules in the solutions were used to build up sequential and very thin bilayers on the modified gold surface. The effects of NaCl and graphene oxide (GO) addition on the mass of the deposited films were also investigated. Intermolecular interactions between the biopolymer chains, Na +  cations and GO sheets were explained by interactions between molecules, such as electrostatic forces and hydrogen bondings.

A Quartz Crystal Microbalance (QCM) Study on the Formation of Aqueous κ-Carrageenan-Chitosan Composite Bilayers with NaCl and Graphene Oxide