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We describe a rapid method for detection of telomeric DNA on chromosomes in the cat. Oligonucleotides were annealed to complementary sequences in fixed metaphase chromosomes of the cat and extended using Taq-DNA polymerase. Digoxygenin-11-dUTP was incorporated as a label for detection of the newly synthesized product. This method represents a quick and reliable alternative to conventional fluorescence in situ hybridization.

Detection of repetitive telomeric DNA on chromosomes in the cat by primed in situ labelling (PRINS)