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Summary The karyotype of Carollia perspicillata (2n = 20/XX and 2n = 21XY1 Y2 was analyzed by C-banding and staining with fluorochromes. Cbanding revealed heterochromatin blocks in the pericentromeric regions of all chromosomes, in the telomeric regions of the short arms of chromosomes 2, 3, 5, X, and in the interstitial region of the short arm of chromosome 1. The Y1 chromosome showed a small block in the pericentromeric region and Y2 was found to be almost completely heterochromatic. Slides treated for C-banding and stained with DAPI/DA or DAPI/AMD (CB-DAPI) showed a heterochromatin block pattern similar to that obtained by C-banding. Triple staining with CMA3/DA/DAPI revealed CMA3-positive blocks in the pericentromeric regions of chromosomes 1, 2, 3, 4 and 5, and DAPI-positive blocks in the interstitial region of the short arm of chromosome 1 and in the telomeric regions of the short arms of chromosomes 2, 3 and 5. Triple staining also showed Rand G-banding patterns rich in GC and AT base pairs, respec...

caryologia

Characterization of the constitutive heterochromatin of <i>Carollia perspicillata</i> (Phyllostomidae, Chiroptera) using the base-specific fluorochromes, CMA<sub>3</sub> (GC) and DAPI (AT)

Characterization of the constitutive heterochromatin of Carollia perspicillata (Phyllostomidae, Chiroptera) using the base-specific fluorochromes, CMA3 (GC) and DAPI (AT)

Characterization of the constitutive heterochromatin of Carollia perspicillata (Phyllostomidae, Chiroptera) using the base-specific fluorochromes, CMA₃ (GC) and DAPI (AT)