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1. An experiment was conducted with White Leghorn layers (Babcock BV 300) to optimise the feed withdrawal schedule for egg-laying hens (67 to 80 weeks) reared in open-sided poultry houses during the tropical summer season. 2. Four feeding schedules (1.  ad libitum  access to the layer feed, 2. feed withdrawal from 9 AM-5 PM, 3. 11 AM-5 PM, and 4. 1 PM-5 PM followed by free access to the layer feed) were examined. Each feeding schedule was offered to 12 replicates consisting of 12 layers housed in four adjacent cages (45.7 × 35.6 × 43.2 cm). 3. Fasting the layers from 1-5 pm reduced egg production compared to layers fasting from 9 or 11 am. The FI/egg or unit egg mass was highest in layers fasted from 1-5 pm. In the 9 am-5 pm fasted group, the cloacal temperature (CT) increased significantly at 11 am and gradually reduced at 3 pm. In the 1-5 pm fasted group, the CT remained higher at 3 pm and 5 pm. 4. The cell-mediated immune response in layers fasted from 9 am or 11 am was higher than those fed  ad libitum  or 1-5 pm fasted group. Lipid peroxidation was higher in layers 1-5 pm fasted group, and significantly lower LP was found in groups that fasted from 9 am or 11 am. In general, the activities of serum antioxidant enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase) were higher in layers that fasted from 9 am or 11 am compared to those that had  ad libitum  feed or fasted from 1-5 pm. 5. It was concluded that the feed withdrawal should be initiated during the forenoon (9 AM/11 am-5 pm) for optimum production and to reduce oxidative stress in egg-laying hens reared during summer in the tropical region.

Feed withdrawal influences egg production, egg quality and antioxidant variables in White Leghorn layers reared during the tropical summer season