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A novel electrochemical sensing platform based on Fe 3 O 4 -antibody conjugate was developed for the rapid and sensitive determination of  Salmonella typhimurium  antigen in model suspensions. The conjugates Fe 3 O 4 -antibody were synthesized  via  carbodiimide cross-linking and characterized by infrared spectroscopy and cyclic voltammetry. The electrochemical properties of Fe 3 O 4  magnetic nanoparticles in water were investigated; the possible mechanism of direct electrochemical reduction was proposed and justified as an analytical signal. The design of an electrochemical sensing platform with magnetically controlled flows has been proposed. Under the most appropriate conditions, the developed portable device was provided the linear response as  I  (µA) = (7.20 ± 0.35) × (–lg C Ag ) − (23.77 ± 1.95) in the concentration range 1 × 10 −7 –1 × 10 −4  mg/mL. The detection limit was 390 ng/mL and was comparable to other works.

analytical letters

Novel electrochemical immunosensing platform based on magnetite-antibody conjugate as a direct signal label: design and application for  Salmonella typhimurium  antigen determination