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A novel poly(diallyldimethylammonium chloride) (PDDA) surface modified magnetic cobalt ferrite-filled multiwalled carbon nanotubes (CoFe 2 O 4 @MWCNTs-PDDA) composite is reported. The CoFe 2 O 4 @MWCNTs-PDDA was characterized by transmission electron microscopy, fourier transform infrared spectrometry, x-ray diffraction, and vibrating sample magnetometry and used for the selective adsorption of acidic proteins. The presence of MWCNTs served as a protective layer, which improved the stability of the magnetic composite, and also enlarged the surface area from 67 m 2 ·g −1  to 186 m 2 ·g −1 , which provided a substrate with larger specific surface area for PDDA wrapping and enhanced the selectivity and adsorption capacity for acidic proteins. The adsorption capacity of CoFe 2 O 4 @MWCNTs-PDDA was 378.1 mg·g −1  for bovine serum albumin and 363.4 mg·g −1  for ovalbumin, an increase of approximately 20-fold, 3-fold, and 4-fold compared to CoFe 2 O 4 , CoFe 2 O 4 @PDDA and CoFe 2 O 4 @MWCNTs, respectively. The practical application of the CoFe 2 O 4 @MWCNTs-PDDA was demonstrated by processing whole blood samples for the extraction of acidic proteins. Satisfactory results were obtained by sodium dodecyl sulfate–polyacrylamide gel electrophoresis.

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Selective Separation and Adsorption of Bovine Serum Albumin and Ovalbumin by Magnetic Solid-Phase Extraction (MSPE) Using Poly(Diallyldimethylammonium Chloride) Modified Cobalt Ferrite Filled Multiwalled Carbon Nanotubes as the Adsorbent