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Alkaline phosphatase (ALP) is a significant biomarker of diseases, such as osteoblastic bone cancer, hepatitis, and myeloma, and hence its determination is essential and important. MnO 2 /Mn 2+  conversion-based magnetic relaxation sensing was developed to directly determine ALP in blood. In the presence of ALP, 2-phospho-L-ascorbic acid trisodium salt was catalyzed to produce ascorbic acid (AA), which reduced MnO 2  to Mn 2+ , causing transverse relaxation time (T 2 ) decay. A linear relationship of ALP from 5 mU·L −1  to 500 mU·L −1  with T 2  signal and a low limit of detection (LOD) of 3.32 mU·L −1  were obtained using the optimized conditions of 200 µM of ascorbic acid-phosphate (AAP), 10 µg·mL −1  of MnO 2 , 37 °C, pH 7.4, and a 30 min reaction time. When directly measuring ALP in serum with the proposed method, the recoveries were from 99.2% to 101.3%, in accordance with values obtained by a commercial assay. In conclusion, a sensitive one-step method for ALP in complex samples was established which has potential for clinical applications.

analytical letters

One-Step Determination of Alkaline Phosphatase in Human Serum Based on Manganese (IV) Dioxide/Manganese (II)-Mediated Nuclear Magnetic Resonance (NMR) Relaxation