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A sensitive and selective method for determining chloramphenicol (CAP) based on RecJ f  exonuclease-assisted signal amplification and graphdiyne (GDY) is reported. GDY possesses a strong absorptivity for single-stranded DNA and is proposed for the first time as a new specific and eminently sensitive type of fluorescence sensing platform for chloramphenicol. Under the optimized conditions, the assay operates from 10 to 80 µg/L chloramphenicol with a 43.6 ng/L detection limit. The procedure was employed to determine chloramphenicol in food samples, with recoveries from 93.60% to 107.90%. The proposed technique offers a novel development in food safety using sensitive aptasensors for detecting small molecules.

Sensitive Fluorescent Determination of Chloramphenicol Based upon Graphdiyne and RecJ f Exonuclease-Assisted Signal Amplification