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The indiscriminate use of organophosphorus pesticides poses a great threat to human health because of harmful substance bioaccumulation. We developed a rapid and reliable method for the simultaneous determination of chlorpyrifos, isocarbophos, methamidophos, omethoate, and triazophos in procine hair. The targets were separated by a C 18  column (2.1 × 100 mm, 1.8 µm) and analyzed by electrospray ionization using the positive mode of a triple quadrupole mass spectrometer. The analytes had linear relationships from 1.0 to 100 µg/kg. The correlation coefficients were all greater than 0.998. The limits of detection ranged from 0.4 to 0.9 µg/kg, and the limits of quantification from 1.2 to 2.0 µg/kg. The recoveries were from 76.1% to 106.2%. The intra-assay and inter-assay precision rates were from 1.6% to 4.4% and 2.3% to 6.9%, respectively. The intra-day and inter-day accuracy ranged from 2.5% to 9.3% and 3.2% to 8.9%, respectively. This method was a valid tool to monitor organophosphorus pesticides in breeding and livestock products.

analytical letters

Determination of Organophosphorus Pesticides in Porcine Hair by a QuEChERS Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry Protocol