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A rapid fluorescence sensor was developed for the determination of DNA using trimetallic zinc nickel aluminum-layered double hydroxides as the fluorescence quencher and carboxytetramethylrhodamine (TAMRA) fluorophore-labeled DNA as the probe. The anion exchangeability of trimetallic zinc nickel aluminum-layered double hydroxides allowed the adsorption of the TAMRA-labeled DNA probes which resulted in the quenching of TAMRA. When the target DNA was in the system, it bound with the complementary TAMRA-labeled DNA to form double-stranded DNA away from the quenchers, thereby preventing the quenching. The developed method provides high sensitivity for DNA from 2 to 20 nM with a detection limit of 0.45 nM within 15 min. This simple and efficient approach is versatile for DNA and demonstrates the application of layered double hydroxides in biosensing.

Rapid Fluorescent Determination of DNA Based on Trimetallic Zinc Nickel Aluminum-Layered Double Hydroxides and Carboxytetramethylrhodamine