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The goal of the current work was to study the phytochemical content of  Onosma inexspectata  Teppner and  O. armenum  DC. The antioxidant properties using six assays and the enzyme inhibitory capacities against α-amylase, α-glucosidase, tyrosinase, and the anti-cholinesterase were also evaluated. The levels of phenolic compounds were approximately 22 and 28 mg gallic acid equivalent/g for  O. inexspectata  and  O. armenum , respectively. Twenty-seven compounds were identified and quantified using liquid chromatography–electrospray ionization tandem mass spectrometry. Chlorogenic acid, apigenin 7-glucoside, and luteolin 7-glucoside were the major compounds in  O. inexspectata .  Onosma armenum  contained an attractive level of hesperidin. The antioxidant assays showed significant differences between the two species, except for the phosphomolybdenum assay. The results of the inhibitory enzyme assays revealed no significant differences between the extracts, except for α-glucosidase inhibition. The present study may underline the possibility to use  O. inexspectata  and  O. armenum  as promising sources of phytochemicals that may serve against many body disorders.

analytical letters

Onosma inexspectata  and  Onosma armenum  as Novel Sources of Phytochemicals with Determination by High-Performance Liquid Chromatography–Mass Spectrometry (HPLC-MS/MS) with Evaluation of the Antioxidant and Enzyme Inhibitory Capacities