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To the best of our knowledge, this is the first study on the effect of solvent (water, methanol, and ethyl acetate) upon phenolics extracted from  Onosma mitis . The antioxidant capacities together with the enzyme inhibitory properties toward tyrosinase and α-amylase were also assessed. The yields of the extract were 2.62, 10.93, and 21.68% for ethyl acetate, methanol, and water, respectively. The concentrations of phenolic compounds were from 11.89 (ethyl acetate) to 2.66 mg gallic acid equivalent/g extract (methanol). Twenty-five molecules were identified and quantified by liquid chromatography – electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Significant differences were present between the extracts; methanol presented the highest number of compounds (25) while ethyl acetate provided the lowest (18). Chlorogenic acid, luteolin 7-glucoside, rosmarinic acid, and apigenin 7-glucoside were the major compounds regardless of solvent. Antioxidant and inhibitory enzyme assays showed that O.  mitis  exhibited interesting biological properties. The results illustrate the use of O.  mitis  as a promising source of biomolecules for industrial applications.

analytical letters

Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS) Identification of Phytochemicals and the Effects of Solvents on Phenolic Constituents, Antioxidant Capacity, Skin-Whitening and anti-Diabetic Activity of  Onosma mitis