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To compare self-rated orthodontic pain (OP) and whole salivary alpha-amylase (αA) and cortisol levels (CL) during early stages of fixed orthodontic and clear aligner therapy (CAT). In groups 1 and 2, malocclusions were treated using fixed orthodontic appliances and CAT, respectively. In Group-3, individuals had normal occlusion and had never undergone orthodontic therapy. Self-rated OP was assessed using the visual-analogue-scale at baseline (T0); after 24-hours (T1) of appliance activation; and after 30 days (T2). Unstimulated whole saliva was collected and αA and CL were measured using enzyme-linked immunosorbent assay.  p  &lt; .01 was considered statistically significant. Twenty-four (Group-1), 24(Group-2) and 25 (Group-3) patients were included. In groups 1 and 2, participants had Class-I malocclusion with anterior-crowding in both arches. At baseline (T0) none of the participants reported pain on mastication. In groups 1 ( p  &lt; .01) and 2 ( p  &lt; .01), OP was higher at T1 than T2. In groups 1 and 2, αA and CL were higher at T1 ( p  &lt; .01) than T0 and T2. At T1 and T2, salivary αA and CL were higher in groups 1 ( p  &lt; .01) and 2 ( p  &lt; .01) than Group-3. In groups 1 and 2, a significant correlation was recorded between OP and αA ( p  &lt; .01) and CL ( p  &lt; .01) at T1 interval. Self-rated OP and salivary αA and CL during the early stages of fixed OT and CAT are similar. Whole salivary αA and CL and OP and are high during the first 24 hours of fixed OT and CAT activation.

Comparison of self-rated pain and salivary alpha-amylase and cortisol levels during early stages of fixed orthodontic and clear aligner therapy