Open AccessRapid induction of senescence in human cervical carcinoma cells
Author(s) -
Edward C. Goodwin,
Eva Yang,
Chan-Jae Lee,
HanWoong Lee,
Daniel DiMaio,
Eun-Seong Hwang
Publication year - 2000
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.97.20.10978
Subject(s) - senescence , telomerase , bovine papillomavirus , biology , telomere , cell culture , cell , cell cycle , phenotype , oncogene , microbiology and biotechnology , cancer research , cervical carcinoma , cervical cancer , cancer , gene , genetics , genome
Expression of the bovine papillomavirus E2 regulatory protein in human cervical carcinoma cell lines repressed expression of the resident human papillomavirus E6 and E7 oncogenes and within a few days caused essentially all of the cells to synchronously display numerous phenotypic markers characteristic of cells undergoing replicative senescence. This process was accompanied by marked but in some cases transient alterations in the expression of cell cycle regulatory proteins and by decreased telomerase activity. We propose that the human papillomavirus E6 and E7 proteins actively prevent senescence from occurring in cervical carcinoma cells, and that once viral oncogene expression is extinguished, the senescence program is rapidly executed. Activation of endogenous senescence pathways in cancer cells may represent an alternative approach to treat human cancers.
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